H2N2V1, Main, Exploration, bibRecord, 001863

Reverse Genetics of Influenza Virus

Identifieur interne : 001863 ( Main/Exploration ); précédent : 001862; suivant : 001864

Reverse Genetics of Influenza Virus

Auteurs : Gabriele Neumann [États-Unis] ; Yoshihiro Kawaoka [États-Unis]

Source :

Virology [ 0042-6822 ] ; 2001.

RBID : ISTEX:006A24C73957C8CD5632F82ED43CC65EF3222D17

English descriptors

Teeft :
- Acad, Cdna, Cdna encoding, Enami, Encoding, Foreign gene, Foreign protein, Genetics, Genetics system, Genetics systems, Genome, Helper virus, Hobom, Influenza, Influenza particles, Influenza virus, Influenza viruses, Kawaoka, Minireview, Mrna, Mutation, Natl, Neumann, Nuclear export, Palese, Plasmid, Polymerase, Polymerase system, Proc, Promoter, Protein expression, Recombinant, Recombinant influenza virus, Recombinant virus, Replication, Rna, Terminator, Terminator sequences, Transcription, Transfected, Transfection, Vaccine, Viral, Viral proteins, Viral rnas, Virol, Virology, Virus, Virus generation, Vrna, Vrnp.

Abstract

Abstract: Reverse genetics of negative-sense RNA viruses, which enables one to generate virus entirely from cloned cDNA, has progressed rapidly over the past decade. However, despite the relative ease with which nonsegmented negative-sense RNA viruses can now be produced from plasmids, the ability to generate viruses with segmented genomes has lagged considerably, largely because of the inherent technical difficulties in providing all viral RNAs and proteins from cloned cDNA. A breakthrough in reverse genetics technology in the influenza virus field came in 1999, when we (Neumann et al., 1999, Proc. Natl. Acad. Sci. USA 96, 9345–9350) and others (Fodor et al., 1999, J. Virol. 73, 9679–9682) exploited a new approach to viral RNA production. In this review, we discuss the background for this advance, the systems that are now available for the generation of influenza viruses, and the implications of these developments for the future of virus research.

Url:

https://api.istex.fr/ark:/67375/6H6-CN3R1LMH-M/fulltext.pdf

DOI: 10.1006/viro.2001.1008

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000F33
to stream Istex, to step Curation: 000F33
to stream Istex, to step Checkpoint: 000690
to stream Main, to step Merge: 001903
to stream Main, to step Curation: 001863

Le document en format XML

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<term>Foreign gene</term>
<term>Foreign protein</term>
<term>Genetics</term>
<term>Genetics system</term>
<term>Genetics systems</term>
<term>Genome</term>
<term>Helper virus</term>
<term>Hobom</term>
<term>Influenza</term>
<term>Influenza particles</term>
<term>Influenza virus</term>
<term>Influenza viruses</term>
<term>Kawaoka</term>
<term>Minireview</term>
<term>Mrna</term>
<term>Mutation</term>
<term>Natl</term>
<term>Neumann</term>
<term>Nuclear export</term>
<term>Palese</term>
<term>Plasmid</term>
<term>Polymerase</term>
<term>Polymerase system</term>
<term>Proc</term>
<term>Promoter</term>
<term>Protein expression</term>
<term>Recombinant</term>
<term>Recombinant influenza virus</term>
<term>Recombinant virus</term>
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<term>Rna</term>
<term>Terminator</term>
<term>Terminator sequences</term>
<term>Transcription</term>
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<term>Transfection</term>
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<term>Viral proteins</term>
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<term>Virus</term>
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<front><div type="abstract" xml:lang="en">Abstract: Reverse genetics of negative-sense RNA viruses, which enables one to generate virus entirely from cloned cDNA, has progressed rapidly over the past decade. However, despite the relative ease with which nonsegmented negative-sense RNA viruses can now be produced from plasmids, the ability to generate viruses with segmented genomes has lagged considerably, largely because of the inherent technical difficulties in providing all viral RNAs and proteins from cloned cDNA. A breakthrough in reverse genetics technology in the influenza virus field came in 1999, when we (Neumann et al., 1999, Proc. Natl. Acad. Sci. USA 96, 9345–9350) and others (Fodor et al., 1999, J. Virol. 73, 9679–9682) exploited a new approach to viral RNA production. In this review, we discuss the background for this advance, the systems that are now available for the generation of influenza viruses, and the implications of these developments for the future of virus research.</div>
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Reverse Genetics of Influenza Virus

Reverse Genetics of Influenza Virus

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri